Identification of 14-3-3-like protein in sugarcane (Saccharum officinarum)

Abstract

In a search of the sugarcane expressed sequence tag (SUCEST) database we located three full-length cDNAs (SCCCLR1022D05.g, SCCCRZ1001D02.g and SCBFLR1026E02.g) encoding the 14-3-3 proteins from sugarcane (Saccharum officinarum). The encoded proteins were identified based on the clustering of the expressed sequence tags and were shown to encode proteins similar to 14-3-3 proteins of other monocotyledonous plants. Cluster SCCCLR1022D05.g was 99% similar to the maize 14-3-3-like protein (gi|1345587) while cluster SCCCRZ1001D02.g shared 96% and SCBFLR1026E02.g 94% similarity with the 14-3-3 protein of rice (gi|7435022). Although 14-3-3 proteins have been reported to be specific to particular species, tissue or organ from which they were isolated, all three sugarcane clusters were found to be expressed in several tissues.Seqüências completas de três cDNAs que codificam proteínas 14-3-3 de cana-de-açúcar (Saccharum officinarum) foram encontradas no projeto genoma EST de cana-de-açúcar. As proteínas codificadas por essas seqüências foram identificadas baseando-se no agrupamento de ESTs de cana-de-açúcar e, três "clusters" (SCCCLR1022 D05.g, SCCCRZ1001D02.g e SCBFLR1026E02.g) foram similares a proteínas 14-3-3 de outras monocotiledôneas. O cluster SCCCLR1022D05.g apresentou similaridade de 99% com a proteína 14-3-3 de milho (gi|1345587) e, os clusters SCCCRZ1001D02.g e SCBFLR1026E02.g foram similares a proteína 14-3-3 de arroz (gi|7435022), 96% e 94%, respectivamente. Embora proteínas 14-3-3 têm sido relatadas como sendo específicas a organismos, tecidos e órgãos, todos os "clusters" de cana-de-açúcar correspondentes a 14-3-3 foram provenientes de cDNAs advindos de diferentes tecidos.