P32 Salvia hispanica L. (chia) seed promotes the reduction of body fat and improves lipid metabolism disorders in the white adipose tissue of rats fed a sucrose rich diet

Abstract

Introduction: In recent years, several studies have focused on the mechanisms involved in the accumulation of visceral fat and its role in the pathophysiology of Metabolic Syndrome, in order to find therapeutic strategies for its prevention and/or treatment. In this sense, the Salvia hispanica L. (chia) seed has generated great interest as a source of bioactive compounds (α-linolenic acid, fibers, high-quality proteins, vitamins, minerals and antioxidants). Objectives: To analyze the effects of chia seed on the metabolic pathways that play a key role in white adipose tissue lipids handling and that could be involved in the reduction of visceral adiposity developed in rats fed a sucrose rich diet (SRD). Materials and methods: Male Wistar rats were fed a reference diet (RD) -6 months- or a SRD -3 months. Then the last group was randomly divided into two subgroups. One subgroup continued to receive SRD until 6 months and the other received a SRD where whole chia seed was incorporated as a source of fat for the next 3 months (SRD+CHIA). At the end of the experimental period, the following were analyzed in epididymal adipose tissue: lipogenic (ATP citrate lyase ATP CL, Fatty Acid Synthase FAS, Phosphoenol Pyruvate carboxykinase PEPCK, Malic Enzyme EM and Acetyl CoA carboxylase ACC) and oxidative (Carnitine Pamitoyl transferase CPT) enzymes activities, protein mass levels of FAT/CD 36, SREBP-1c, totalAMPK, pAMPK and PKCβ. In addition, biometrical parameters, energy intake, carcass composition, serum lipids, visceral adiposity, and lipid content in adipocytes were determined. Euglycemic-hyperinsulinemic clamp.

Keywords

• chía
• reducción grasa corporal
• mejora desórdenes metabolismo lipídico