Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

Guy Prod'hom; Christian Durussel; Dominique Blanc; Antony Croxatto; Gilbert Greub

doi:10.1016/j.nmni.2015.05.007

New Microbes and New Infections (Nov 2015)

Early detection of extended-spectrum β-lactamase from blood culture positive for an Enterobacteriaceae using βLACTA test

Guy Prod'hom,
Christian Durussel,
Dominique Blanc,
Antony Croxatto,
Gilbert Greub

Affiliations

Guy Prod'hom: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
Christian Durussel: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
Dominique Blanc: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
Antony Croxatto: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland
Gilbert Greub: Institute of Microbiology, University of Lausanne and University Hospital Center, Lausanne, Switzerland

DOI: https://doi.org/10.1016/j.nmni.2015.05.007
Journal volume & issue: Vol. 8, no. C
pp. 1 – 3

Abstract

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Bacterial pellets from Enterobacteriaceae positive blood cultures prepared using ammonium chloride were tested for rapid detection of β-lactamase using the commercial βLACTA test and read after 30 minutes. During 7 months, 137 bacterial pellets were tested prospectively. βLACTA test exhibited a sensitivity of 75% and a specificity of 100% for the detection of third-generation cephalosporin resistance. False negative tests were mainly observed with hyperproduced chromosomal or plasmid-borne AmpC.

Published in New Microbes and New Infections

ISSN: 2052-2975 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Medicine: Internal medicine: Infectious and parasitic diseases
Website: https://www.sciencedirect.com/journal/new-microbes-and-new-infections

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