Tropical Medicine and Infectious Disease (Mar 2023)

Drug Resistance Molecular Markers of <i>Plasmodium falciparum</i> and Severity of Malaria in Febrile Children in the Sentinel Site for Malaria Surveillance of Melen in Gabon: Additional Data from the Plasmodium Diversity Network African Network

  • Jacques Mari Ndong Ngomo,
  • Denise Patricia Mawili-Mboumba,
  • Noé Patrick M’Bondoukwé,
  • Bridy Moutombi Ditombi,
  • Jeanne Vanessa Koumba Lengongo,
  • Fanny Bertrande Batchy Ognagosso,
  • Marielle Karine Bouyou-Akotet

DOI
https://doi.org/10.3390/tropicalmed8040184
Journal volume & issue
Vol. 8, no. 4
p. 184

Abstract

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The objective of this study was to analyze the relationship between the frequency of artemisinin-based combination (ACT) drug resistance molecular markers and clinical forms of P. falciparum malaria and parasitemia. A cross-sectional study was carried out between January and April 2014 at the Operational Clinical Research Unit of Melen in febrile children aged 12 to 240 months with a Plasmodium sp. infection. A total of 3 mL of peripheral blood collected from an EDTA tube was used for leukocyte depletion. DNA mutation detection was performed by next generation sequencing (NGS). A total of 1075 patients were screened for malaria. Among them, 384 had a Plasmodium infection. P. falciparum mono-infection was found in 98.9% of the patients. Pfcrt-326T mutation was found in all isolates, while 37.9% had Pfmdr2-484I mutant allele. The highest median parasite densities were found in patients infected by parasites carrying the CVIET haplotype of the Pfcrt gene. The different genetic profiles found here, and their variations according to clinical and biological signs of severe malaria, are additional arguments for the surveillance of P. falciparum strains.

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