Frontiers in Genetics (Jun 2022)

BSA-seq Identifies a Major Locus on Chromosome 6 for Root-Knot Nematode (Meloidogyne graminicola) Resistance From Oryza glaberrima

  • Gurwinder Kaur,
  • Inderjit Singh Yadav,
  • Dharminder Bhatia,
  • Yogesh Vikal,
  • Kumari Neelam,
  • Narpinderjeet Kaur Dhillon,
  • Umesh Preethi Praba,
  • Gurjit Singh Mangat,
  • Kuldeep Singh,
  • Kuldeep Singh

DOI
https://doi.org/10.3389/fgene.2022.871833
Journal volume & issue
Vol. 13

Abstract

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Root-knot nematode (Meloidogyne graminicola) is one of the emerging threats to rice production worldwide that causes substantial yield reductions. There is a progressive shift of the cropping system from traditional transplanting to direct-seeded water-saving rice production that favored the development of M. graminicola. Scouting and deploying new resistance genes is an economical approach to managing the root-knot nematodes. Here, we report that the inheritance of root-knot nematode resistance in Oryza glaberrima acc. IRGC102206 is governed by a single dominant gene. Traditional mapping coupled with BSA-seq is used to map nematode resistance gene(s) using the BC1F1 population derived from a cross of O. sativa cv. PR121 (S) and O. glaberrima acc. IRGC102206 (R). One major novel genomic region spanning a 3.0-Mb interval on chromosome 6 and two minor QTLs on chromosomes 2 and 4 are the potential genomic regions associated with rice root-knot nematode resistance. Within the QTL regions, 19 putative candidate genes contain 81 non-synonymous variants. The detected major candidate region could be fine mapped to accelerate marker-assisted breeding for root-knot nematode resistance in rice.

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