Estimating genetic diversity among durum wheat (Triticum durum desf.) landraces using morphological and SRAP markers

Abstract

The durum wheat (Triticum turgidum L. ssp. durum Desf.) Landraces are a valuable source of natural germplasm for enhancing the genetic diversity of existing durum wheat cultivars. The study analyzed the genetic diversity of twenty-two durum wheat landraces including eight local Saudi landraces and fourteen introduced landraces using sixteen morphological descriptors and sequence-related amplified polymorphism (SRAP) markers. The results showed significant differences between the analyzed agro-morphological parameters and a large degree of genetic variation across the tested landraces. Principle components analysis (PCA) was used to determine agro-morphological relationships among different traits. The first six principal components (PCs) accounted for 78.47 % of the cumulative variability among 22 landraces. The PC1demonstrated variability in coleoptile anthocyanin coloration, straw pith, culm neck glaucosity, flag leaf glaucosity, ear glaucosity, and length of awn at tip relative to length of ear. Wheat landraces (L8, L14, L497, L569, L590, L594, L600, L601, and L602) showed increased diversity in dry region and contributed to PC1. A couple landraces had higher plant height (L12, L598, and L654), heading days (650, 656), and ear length (L12, L594, and L650) respectively. The dendrogram generated from the standardized morphological data separated the twenty-two durum wheat genotypes mainly into two clusters. The landraces 14, 600 and 602 grouped into separate cluster with strong correlation to ear glaucosity. Genetic diversity of these landraces was also evaluated using eight SRAP markers, with 482 amplified fragments and an average polymorphic information content (PIC) of 0.27. The dendrogram based on SRAP markers separated into two clusters with some diversified landraces (L8, 594, 602, 977, and L650). The Mantel test found no significant correlation (phenotypic or genetic) between 22 wheat durum landraces (r = 0.013, p = 0.84, =0.05). The results demonstrated the power of morphological and SRAP markers for detecting diversity among durum wheat landraces in the semi-arid region of Saudi Arabia. Thus, study provides insights into wheat diversity for better in-situ and ex-situ conservation and paves the road for their use in a Saudi durum wheat breeding program.

Keywords

• Durum wheat
• Morphological descriptors
• PCA
• Cluster analysis
• SRAP
• Genetic diversity