Nature Communications (May 2022)
A fluorogenic probe for granzyme B enables in-biopsy evaluation and screening of response to anticancer immunotherapies
- Jamie I. Scott,
- Lorena Mendive-Tapia,
- Doireann Gordon,
- Nicole D. Barth,
- Emily J. Thompson,
- Zhiming Cheng,
- David Taggart,
- Takanori Kitamura,
- Alberto Bravo-Blas,
- Edward W. Roberts,
- Jordi Juarez-Jimenez,
- Julien Michel,
- Berber Piet,
- I. Jolanda de Vries,
- Martijn Verdoes,
- John Dawson,
- Neil O. Carragher,
- Richard A. O’ Connor,
- Ahsan R. Akram,
- Margaret Frame,
- Alan Serrels,
- Marc Vendrell
Affiliations
- Jamie I. Scott
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Lorena Mendive-Tapia
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Doireann Gordon
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Nicole D. Barth
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Emily J. Thompson
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Zhiming Cheng
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- David Taggart
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Takanori Kitamura
- MRC Centre for Reproductive Health, Queen’s Medical Research Institute, The University of Edinburgh
- Alberto Bravo-Blas
- Cancer Research UK Beatson Institute
- Edward W. Roberts
- Cancer Research UK Beatson Institute
- Jordi Juarez-Jimenez
- EaStChem School of Chemistry, Joseph Black Building, The University of Edinburgh
- Julien Michel
- EaStChem School of Chemistry, Joseph Black Building, The University of Edinburgh
- Berber Piet
- Department of Pulmonary Diseases, Radboud University Medical Centre
- I. Jolanda de Vries
- Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre
- Martijn Verdoes
- Department of Tumor Immunology, Radboud Institute for Molecular Life Sciences, Radboud University Medical Centre
- John Dawson
- Cancer Research UK Edinburgh Centre, The University of Edinburgh
- Neil O. Carragher
- Cancer Research UK Edinburgh Centre, The University of Edinburgh
- Richard A. O’ Connor
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Ahsan R. Akram
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Margaret Frame
- Cancer Research UK Edinburgh Centre, The University of Edinburgh
- Alan Serrels
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- Marc Vendrell
- Centre for Inflammation Research, Queen’s Medical Research Institute, The University of Edinburgh
- DOI
- https://doi.org/10.1038/s41467-022-29691-w
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 11
Abstract
Granzyme B is found in activated T cells and can be used as a marker of T cell activation. Here, the authors generate a fluorescent probe that can detect Granzyme B levels in tumours, and has the potential to be used as a biomarker of response to immunotherapy.
