Viruses (Aug 2019)

Middle East Respiratory Syndrome Coronavirus (MERS-CoV) in Dromedary Camels in Africa and Middle East

  • Ahmed Kandeil,
  • Mokhtar Gomaa,
  • Ahmed Nageh,
  • Mahmoud M. Shehata,
  • Ahmed E. Kayed,
  • Jamal S. M. Sabir,
  • Awatef Abiadh,
  • Jamel Jrijer,
  • Zuhair Amr,
  • Mounir Abi Said,
  • Denis K. Byarugaba,
  • Fred Wabwire-Mangen,
  • Titus Tugume,
  • Nadira S. Mohamed,
  • Roba Attar,
  • Sabah M. Hassan,
  • Sabah Abdulaziz Linjawi,
  • Yassmin Moatassim,
  • Omnia Kutkat,
  • Sara Mahmoud,
  • Ola Bagato,
  • Noura M. Abo Shama,
  • Rabeh El-Shesheny,
  • Ahmed Mostafa,
  • Ranawaka A. P. M. Perera,
  • Daniel K. W. Chu,
  • Nagla Hassan,
  • Basma Elsokary,
  • Ahmed Saad,
  • Heba Sobhy,
  • Ihab El Masry,
  • Pamela P. McKenzie,
  • Richard J. Webby,
  • Malik Peiris,
  • Yilma J. Makonnen,
  • Mohamed A. Ali,
  • Ghazi Kayali

DOI
https://doi.org/10.3390/v11080717
Journal volume & issue
Vol. 11, no. 8
p. 717

Abstract

Read online

Dromedary camels are the natural reservoirs of the Middle East respiratory syndrome coronavirus (MERS-CoV). Camels are mostly bred in East African countries then exported into Africa and Middle East for consumption. To understand the distribution of MERS-CoV among camels in North Africa and the Middle East, we conducted surveillance in Egypt, Senegal, Tunisia, Uganda, Jordan, Saudi Arabia, and Iraq. We also performed longitudinal studies of three camel herds in Egypt and Jordan to elucidate MERS-CoV infection and transmission. Between 2016 and 2018, a total of 4027 nasal swabs and 3267 serum samples were collected from all countries. Real- time PCR revealed that MERS-CoV RNA was detected in nasal swab samples from Egypt, Senegal, Tunisia, and Saudi Arabia. Microneutralization assay showed that antibodies were detected in all countries. Positive PCR samples were partially sequenced, and a phylogenetic tree was built. The tree suggested that all sequences are of clade C and sequences from camels in Egypt formed a separate group from previously published sequences. Longitudinal studies showed high seroprevalence in adult camels. These results indicate the widespread distribution of the virus in camels. A systematic active surveillance and longitudinal studies for MERS-CoV are needed to understand the epidemiology of the disease and dynamics of viral infection.

Keywords