RELATIONSHIP OF CHANGES IN HEMATOLOGICAL AND BIOCHEMICAL INDICATORS OF PERIPHERAL BLOOD WITH THE TRANSFERRIN CONCENTRATION AND CD71+ LYMPHOCYTE COUNT

Abstract

Transferrin is an essential component of cell growth and metabolic processes that require iron. An increase in the expression of transferrin receptors on lymphocytes ensures their differentiation, maturation and activation of proliferation. It was of interest to study the relationship between the level of transferrin in the peripheral blood and the level of lymphocytes with the receptor to it. Purpose. To determine the nature of changes in hematological and biochemical parameters of peripheral blood based on the concentration of transferrin and the count of lymphocytes expressing the transferrin receptor CD71+. Materials and methods. a survey of 100 people aged 20 to 40 years living in the city of Arkhangelsk was carried out. Leukograms were carried out using a XS-1000i hematology analyzer (Sysmex). Enzyme immunoassay was used to measure cytokine concentrations. CD71 lymphocyte counts were carried out by flow cytometry (Epixs XL). A biochemical analyzer (STAT FAX 3300) was used to measure biochemical blood parameters. Analyses of the number of lymphocytes expressing the transferrin receptor and the concentration of transferrin in the blood serum were performed. The number of CD71+ lymphocytes within the physiological norm (less than 0.3 × 109 cells/L) and those with increased CD71+ levels (more than 0.5 × 109 cells/L) was identified. Within each group, subgroups with a normal and high transferrin content were identified. Results. It was found that an increase in the concentration of transferrin in the blood is associated with a decrease in the circulation of lymphocytes with the CD71+ receptor and a decrease in the coefficient of transferrin saturation with iron. This situation may be associated with an increased risk of secondary immunodeficiency. Against the background of increased concentrations of transferrin and an increase in the circulation of CD71+ lymphocytes, the concentration of lactate increases, which reflects an increase in the processes of tissue hypoxia. Conclusion. High concentrations of transferrin in the peripheral blood against the background of a decrease in the saturation of transferrin with iron and an increase in tissue hypoxia increase the risk of developing chronic inflammatory processes and may be associated with the formation of secondary immunodeficiency.

Keywords

• biochemical indicators of blood
• transferrin
• iron
• receptor
• tfr1
• cd71
• cytokines
• transferrin saturation