In vitro antifungal activity of barberry fruit extract (Berberis spp.) against Fusarium spp.

Abstract

Purpose: Berberis integerrima Bunge and Berberis vulgaris L. are traditional plants known for their many health benefits. The aim of this study was to investigate the antifungal potential of B. vulgaris and B. integerrima fruit extracts against Fusarium spp. pathogens as an environmentally compatible natural antifungal compound. Research methods: The antifungal activity of methanolic fruit extracts of B. vulgaris and B. integerrima against Fusarium solani, and Fusarium graminearum was investigated using the microdilution method, growth area measurement, and morphological Changes were studied using scanning electron microscopy analysis. Findings: The methanolic fruit extracts of B. vulgaris and B. integerrima had significant antifungal activity against the studied plant pathogens, with B. integerrima exhibiting a stronger effect. The MIC values of B. vulgaris fruit extract against F. graminearum and F. solani were 150 and 75 mg mL-1, and B. integerrima fruit extract had 100 and 75mg mL-1, respectively. F. graminearum was the most resistant fungal species. Scanning electron microscopy analysis showed that the extracts of both medicinal plants changed the structure and morphology of mycelia and, dose-dependently, inhibited conidia formation. Research limitations: There were no limitations. Originality/Value: The study showed that fruit extracts of B. vulgaris and B. integerrima have the potential to be used as natural and environmentally friendly agents against Fusarium species.

Keywords

• mycelial morphology
• natural antifungal compound
• plant pathogen
• sem