Nature Communications (Apr 2022)
Light triggered nanoscale biolistics for efficient intracellular delivery of functional macromolecules in mammalian cells
- Juan C. Fraire,
- Elnaz Shaabani,
- Maryam Sharifiaghdam,
- Matthias Rombaut,
- Charlotte Hinnekens,
- Dawei Hua,
- Jana Ramon,
- Laurens Raes,
- Eduardo Bolea-Fernandez,
- Toon Brans,
- Frank Vanhaecke,
- Peter Borghgraef,
- Chaobo Huang,
- Félix Sauvage,
- Tamara Vanhaecke,
- Joery De Kock,
- Ranhua Xiong,
- Stefaan De Smedt,
- Kevin Braeckmans
Affiliations
- Juan C. Fraire
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Elnaz Shaabani
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Maryam Sharifiaghdam
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Matthias Rombaut
- Department of In vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel (VUB)
- Charlotte Hinnekens
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Dawei Hua
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Jana Ramon
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Laurens Raes
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Eduardo Bolea-Fernandez
- Department of Chemistry, Atomic & Mass Spectrometry – A&MS Research Group, Ghent University
- Toon Brans
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Frank Vanhaecke
- Department of Chemistry, Atomic & Mass Spectrometry – A&MS Research Group, Ghent University
- Peter Borghgraef
- VIB Bioimaging Core Ghent, VIB
- Chaobo Huang
- Joint Laboratory of Advanced Biomedical Materials (NFU‐UGent), College of Chemical Engineering, Nanjing Forestry University (NFU)
- Félix Sauvage
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Tamara Vanhaecke
- Department of In vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel (VUB)
- Joery De Kock
- Department of In vitro Toxicology and Dermato-Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel (VUB)
- Ranhua Xiong
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Stefaan De Smedt
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- Kevin Braeckmans
- Laboratory for General Biochemistry and Physical Pharmacy, Faculty of Pharmaceutical Sciences, Ghent University
- DOI
- https://doi.org/10.1038/s41467-022-29713-7
- Journal volume & issue
-
Vol. 13,
no. 1
pp. 1 – 16
Abstract
Ballistic delivery with micro/nano-particles has been successfully used to transfect plant cells, however, has failed in mammalian cells due to toxic effects. Here, the authors report on a self-assembled nano-ballistic delivery system for the delivery of functional macromolecules and demonstrate efficient transfection of mammalian cells.
