An in vivo orthotopic serial passaging model for a metastatic renal cancer study

Abstract

Summary: We developed an in vivo serial passaging model for renal cancer with orthotopic renal subcapsular inoculation. We detail the procedures for renal subcapsular inoculation of cancer cells in mice, followed by in vivo and ex vivo bioluminescence imaging, tumor-bearing kidney dissociation, and in vivo passaging. This protocol is capable of reproducing the coevolution between cancer cells and the primary tumor microenvironment. It enables us to unveil the systemic dynamics of metastasis and develop a therapeutic strategy for metastatic renal cancer.For complete details on the use and execution of this protocol, please refer to Nishida et al. (2020).

Keywords

• Cell Biology
• Cell culture
• Cancer
• Model Organisms