Immunotoxic effects of interferon alpha-2b, modified by immobilization by means of electron-beam synthesis technology

Abstract

The paper presents the results of investigation of immunotoxic action of interferon α-2b, immobilized in polyethylene glycol by means of electron-beam synthesis technology.Materials and methods. In this study we used following methods: a preliminary assessment of immunotoxicity after single administration in wide range of doses, a study of the effect of the drug on the weight and cellularity of central and peripheral immune organs, an assessment of the phagocytic activity of peritoneal macrophages, a study of the effect of the drug on the phagocytic activity of neutrophils, an estimation of the level of serum hemagglutinins to sheep erythrocytes, an evaluation of the effect of the drug on the delayed-type hypersensitivity reaction induced by trinitrobenzenesulfonic acid.Results. We have shown that the course application of the drug based on immobilized interferon α-2b in tenfold and hundredfold therapeutic doses has no immunotoxic action. At the same time, single administration of the drug at various doses and the therapeutic course application in tenfold dose leads to the stimulation of humoral immune responses. The course application of this drug in tenfold and hundredfold therapeutic dose enhances the phagocytic activity of peritoneal macrophages and peripheral blood neutrophils of experimental animals.

Keywords

• интерферон альфа-2b
• пегилирование
• иммунотоксичность
• гуморальный и клеточный иммунный ответ
• фагоцитоз
• мыши-гибриды линии f1(cba×c57bl/6)