Molecules (Jul 2020)

Accumulation of Saponins in Underground Parts of <i>Panax vietnamensis</i> at Different Ages Analyzed by HPLC-UV/ELSD

  • Kim Long Vu-Huynh,
  • Huy Truong Nguyen,
  • Thi Hong Van Le,
  • Chi Thanh Ma,
  • Gwang Jin Lee,
  • Sung Won Kwon,
  • Jeong Hill Park,
  • Minh Duc Nguyen

DOI
https://doi.org/10.3390/molecules25133086
Journal volume & issue
Vol. 25, no. 13
p. 3086

Abstract

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Panax vietnamensis (PV), a wild Panax species discovered in Vietnam in 1973, has been increasingly overexploited due to its economic value and therapeutic uses. This resulted in the development of PV cultivation to meet the market demand. There is little information on the accumulation of saponins in PV during cultivation, but this information could serve as an indication of the appropriate harvest time. In this study we developed an HPLC-UV/ELSD method to simultaneously determine the content of 10 characteristic saponins in PV from 2–7 years old, including G-Rb1, G-Rd, G-Rg1, G-Re, N-R1, M-R1, M-R2, V-R2, V-R11, and p-RT4. The result indicated that from 2 to 5 years, the content of saponins in PV rhizome and radix increase 3.02 and 4.2 times, respectively, whereas from 5 to 7 years, no significant changes were observed. Hence, our study suggests that after 5 years of growth could be considered as an appropriate time for PV to be harvested. Among the analyzed saponins, G-Rg1, G-Rb1, G-Rd, and especially M-R2 were the major saponins that contributed to the change of PV’s saponin content through the years. In addition, the developed and validated HPLC method was proven to be reliable and effective for quality control of PV.

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