Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

D.Y. Zhang; J.Y. Zhou; W.Q. Chen; J.G. Chen

doi:10.17221/23/2009-VETMED

Veterinární Medicína (Apr 2009)

Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization

D.Y. Zhang,
J.Y. Zhou,
W.Q. Chen,
J.G. Chen

Affiliations

D.Y. Zhang: College of Biology and Environmental Eengineering, Zhejiang Shuren University, Hangzhou, China
J.Y. Zhou: Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China
W.Q. Chen: College of Biology and Environmental Eengineering, Zhejiang Shuren University, Hangzhou, China
J.G. Chen: Institute of Preventive Veterinary Medicine, Zhejiang University, Hangzhou, China

DOI: https://doi.org/10.17221/23/2009-VETMED
Journal volume & issue: Vol. 54, no. 4
pp. 169 – 174

Abstract

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The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Vero cells, and the expression of targeted proteins was confirmed by an indirect immunofluorescent assay and immunocytochemistry assay. By repeating intramuscular injection several times, the co-expression plasmids provided chicks with some immune protection against viral infection.

Published in Veterinární Medicína

ISSN: 0375-8427 (Print); 1805-9392 (Online)
Publisher: Czech Academy of Agricultural Sciences
Country of publisher: Czechia
LCC subjects: Agriculture: Animal culture: Veterinary medicine
Website: https://vetmed.agriculturejournals.cz/

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