Vіsnik Naukovih Doslіdžen' (Nov 2017)
THE FEATURES OF MORPHOLOGICAL CHANGES IN BONE TISSUE AFTER USING THE MATERIAL BASED ON HYDROXIAPATITE AND POLYLACTIDE
Abstract
Osteoregeneration in bone defects of jaws restorations is an important issue in modern dentistry. A large number of articles confirm that the ideal material does not exist yet and that is why its search is relevant. The aim of the study – to learn the osteoregeneration processes by morphometric evaluation of micropreparations and conducting a comparative analysis of reparation in correlation with macroscopic data on 180 day of the experiment. Materials and Methods. The experiment was conducted on white male rats weighing 180–200 g. Under general anesthesia animals created a through hole in the jaw area. In the control group, healing was performed only under blood convection, and in the experimental groups, the bone defect was replaced with a hydroxyapatite and polylactide based substance with different percentages of components (group 1 (80 %: 20 %) and group 2 (50 %: 50 %) ) Morphometric evaluation was carried out with using an inserts of an eyepiecemicrometer with measuring grids for cytogystostereometric studies. At macroscopic examination, the overall view of the removed jaws and adjacent tissues was visually assessed. Results and Discussion. In control groups the regeneration with non-mineralized tissue, similar to structure to the organic matrix of the bone was observed. Osteoimplantant with the correlation of polylactide and synthetic hydroxyapatitis 20/80 percentages promotes a smaller tissue destruction, structurally corrects organization of the organic bone matrix and its faster mineralization with the formation of a primary bone marrow for 30 days. At 180th day, the processes of restructuring and transformation of the bone tissue are still quite intense. When applying the implant with the 50/50 percentages, regeneration has a more favorable course, as it was evidenced by the practical restoration of the bone structure for 180 days of observation, when the bulk of the regenerate (70 %) in the defect was represented by a mature lumbar bone. Data obtained in the evaluation of macro drugs of the lower jaws of animals correlated with the data morphometric study. Conclusions. The filling of bone defects by the proposed osteo implants changes the course of regeneration. It was established that filling the defect with a resorbable composite reduces destructive processes and accelerates the formation of the primary and secondary bone mosaic. From this point of view, the composition with the same content of both its components was more effective.
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