Acta Periodica Technologica (Jan 2002)

Determina tion of diacetyl and 2,3-pentanedione in beer by gc/ms using solid-phase extraction columns

  • Pejin Jelena D.,
  • Grujić Olgica S.,
  • Marjanović Nikola J.,
  • Vujić Đura N.,
  • Kocić-Tanackov Sunčica D.

DOI
https://doi.org/10.2298/APT0233045P
Journal volume & issue
Vol. 2002, no. 33
pp. 45 – 54

Abstract

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A new GC/MS method for the determination of diacetyl and 2,3-pentanedione was investigated. Diacetyl and 2,3-pentanedione were derivatized with 1,2-diaminobenzene to form 2,3-dimetylquinoxaline and 2-ethyl-3-methylquinoxaline. respectively. The amounts of formed 2.3-dimetylqu:inoxaline and 2-ethyl-3,.methylquinoxaline were proportional to the concentrations of diacetyl and 2,3-penianedione present in the sample. 2,3-Dimetylquinoxaline and 2-ethyl-3-methylquinoxaline were extracted by solid-phase extraction (SPE) columns and determined by gas chromatography using a mass selective detector. This method was applied for the determination of diacetyl and 2,3-pentanedione concentrations in beer. Extraction by SPE columns proved to be very simple and reliable. The method can be used for simultaneous determination of diacetyl and 2,3-pentanedione concentrations in beer in a great number of beer samples.

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