Veterinary Sciences (Feb 2023)

Analytical Performance Evaluation of the New GEM<sup>®</sup> Premier™ 5000 in Comparison to the Epoc<sup>®</sup> Blood Gas Analyzer in Horses

  • Charlotte Sandersen,
  • Petra Dmitrovic,
  • Julien Dupont,
  • Carla Cesarini,
  • Hugues Guyot,
  • Didier Serteyn,
  • Katharina Kirsch

DOI
https://doi.org/10.3390/vetsci10020114
Journal volume & issue
Vol. 10, no. 2
p. 114

Abstract

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Different blood gas analyzers are used in equine practice. Every machine needs to be validated, as they have not been designed for use in horses. The aim of this study was to compare the newly marketed GEM5000 machine to the formerly validated epoc machine for blood gas analysis in horses. In this prospective, non-blinded, comparative laboratory analyzer study, 43 equine blood samples were analyzed on both analyzers and values were compared between the two machines via Lin’s concordance analysis, Passing–Bablok regression analysis and Bland–Altman plots. Duplicate measurements were conducted on the GEM5000 machine to evaluate precision. The GEM5000 failed to achieve the required precision for tHb, Hct and iCa2+, but achieved acceptable precision for all other parameters. Concordance correlation analysis revealed poor correlation for Na+, Cl−, iCa2+, K+, Hct and tHb, while there was an at least moderate agreement for all other parameters. Passing–Bablok regression revealed significant constant bias for pCO2, pO2, Cl−, and iCa2+ and significant proportional bias for pCO2, iCa2+ and SO2. Bland–Altman analysis revealed significant systematic bias for Na+, Cl−, iCa2+, K+, Hct, tHb and SO2. This study shows that while precision of the GEM5000 is good, values should not be used interchangeably with data obtained from other blood gas analyzers.

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