Significance of TNFRSF11 gene polymorphism in bone resorption reguluation as a pathogenetic factor of yersinia arthritis development

Abstract

Background. СDC (USА) places yersiniosis in ten intestinal infections which are subject to close observation. Exposure to multiple agents is responsible for possible damages of the joints at infection diseases acting as rare forms however, as a pathognomonic syndrome it is found at yersiniosis infections, brucellosis, and Lyme borreliosis. Studies of fine mechanisms of infection arthritis development do gain their relevance. The purpose of the research was to study the TNFRSF11B gene’s Asn3Lys polymorphic marker distribution at patients suffering from generalized and arthritic form of yersiniosis, and to determine the connection of the polymorphism in question with development of yersiniosis arthritis (YA). Materials and methods 123 patients aged 16 to 68 years, with proven yersiniosis infection, were observed. The 1st group included 61 patients with secondary focal arthritic form of yersiniosis whereas the 2nd control group consisted of 62 patients with generalized form of yersiniosis without damaging of joints. 50 patients (82 %) had chronic form of YA. For genetic tests, SNP–express test system – 31Т>С of IL1β gene and Asn3Lys of TNFRSF11B gene was applied. Results. In 2 groups of examined persons, three genotypes being Asn/Asn, Asn/Lys and Lys/Lys were detected. The incidence for genotype Asn/Asn in group with YA made 19.7 %, being 1,7 times lower as compared with control group (33.9 %). The genotype Lys/Lys in group with YA was detected with incidence 31.1 %, which is 2.14 times higher than the value for control group. The genotype Lys/Lys was found prevailing for patients with YA. Genotype Lys/Lys relates to increased risk of development of YA (χ2 = 6.08, р = 0.49; OR = 2.66, confidence interval 1.09–6.49). Coclusions. Polymorphism of Asn3Lys of TNFRSF11B gene coding the osteoprotegerin relates to development of YA. Genotype Lys/Lys of TNFRSF11B gene is a marker of increased risk of development and progressing of YA. Study of genetic predisposition to occurrence of YA, аs well as possible genetically determined resistance to treatment performed based on molecular and genetic testing is an important predictor for YA treatment and prevention.

Keywords

• yersiniosis arthritis
• bone resorption
• osteoprotegerin
• tnfrsf11b gene