Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Irsyad N. A. Khairil Anuar; Anusuya Banerjee; Anthony H. Keeble; Alberto Carella; Georgi I. Nikov; Mark Howarth

doi:10.1038/s41467-019-09678-w

Nature Communications (Apr 2019)

Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox

Irsyad N. A. Khairil Anuar,
Anusuya Banerjee,
Anthony H. Keeble,
Alberto Carella,
Georgi I. Nikov,
Mark Howarth

Affiliations

Irsyad N. A. Khairil Anuar: Department of Biochemistry, University of Oxford, South Parks Road
Anusuya Banerjee: Department of Biochemistry, University of Oxford, South Parks Road
Anthony H. Keeble: Department of Biochemistry, University of Oxford, South Parks Road
Alberto Carella: Department of Biochemistry, University of Oxford, South Parks Road
Georgi I. Nikov: Department of Biochemistry, University of Oxford, South Parks Road
Mark Howarth: Department of Biochemistry, University of Oxford, South Parks Road

DOI: https://doi.org/10.1038/s41467-019-09678-w
Journal volume & issue: Vol. 10, no. 1
pp. 1 – 13

Abstract

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Peptide tags are important for protein purification but have minimal benefits afterwards. Here the authors present Spy&Go, which uses rational engineering of the SpyDock protein to capture SpyTag peptides without requiring an irreverisble isopeptide bond.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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