Generation of human induced pluripotent stem cell line (BCRTi006-A) from a patient with focal segmental glomerulosclerosis disease

Lilas Batool; Constanze Raab; Christien Madlen Beez; Krithika Hariharan; Andreas Kurtz; Maik Gollasch; Bella Rossbach

Stem Cell Research (Jun 2023)

Generation of human induced pluripotent stem cell line (BCRTi006-A) from a patient with focal segmental glomerulosclerosis disease

Lilas Batool,
Constanze Raab,
Christien Madlen Beez,
Krithika Hariharan,
Andreas Kurtz,
Maik Gollasch,
Bella Rossbach

Affiliations

Lilas Batool: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
Constanze Raab: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany
Christien Madlen Beez: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Deutsches Herzzentrum der Charité (DHZC), Department of Cardiothoracic and Vascular Surgery, Augustenburger Platz 1, 13353 Berlin, Germany
Krithika Hariharan: Fraunhofer-Institute for Biomedical Engineering (IBMT), Fraunhofer Project Center for Stem Cell Process Engineering, Neunerplatz 2, 97082 Würzburg, Germany
Andreas Kurtz: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany
Maik Gollasch: Klinik und Poliklinik für Innere Medizin D – Geriatrie, Universitätsmedizin Greifswald, Ferdinand-Sauerbruch-Straße, 17489 Greifswald, Germany
Bella Rossbach: Center for Regenerative Therapies (BCRT), Berlin Institute of Health at Charité – Universitätsmedizin Berlin, Augustenburger Platz 1, 13353 Berlin, Germany; Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany; Corresponding author at: Fraunhofer-Institute for Biomedical Engineering (IBMT), Biomedical Data & Bioethics, Anna-Louisa-Karsch Str. 2, 10178 Berlin, Germany.

Journal volume & issue: Vol. 69
p. 103070

Abstract

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Individuals with transient reception potential cation channel 6 (TRPC6) mutation have variable phenotypes, ranging from healthy carriers to focal segmental glomerulosclerosis (FSGS). Human induced pluripotent stem cell (hiPSC) line was generated from the urinary cells of a patient with FSGS with a mutant variant of TRPC6. The cells were reprogrammed with Yamanaka factors (OCT3, SOX2, LIN28, L-MYC, and KLF4) using a commercially available Epi5 Reprogramming Kit. The pluripotency of the hiPSC line was confirmed by the expression of common stem cell markers and by their ability to generate all germ layers in vitro. The line is available and registered in the human pluripotent stem cell registry as BCRTi006-A. The generated line represents a valuable tool for disease modeling and drug development for FSGS.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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