A study of the protein-protein interactions in the phycocyanin monomer from Synechocystis sp. PCC 6803 using a bacterial two-hybrid system

Simi Shao; Longsheng Zhao; Pingyi Li; Hainan Su; Xiulan Chen; Yuzhong Zhang

Engineering Microbiology (Jun 2022)

A study of the protein-protein interactions in the phycocyanin monomer from Synechocystis sp. PCC 6803 using a bacterial two-hybrid system

Simi Shao,
Longsheng Zhao,
Pingyi Li,
Hainan Su,
Xiulan Chen,
Yuzhong Zhang

Affiliations

Simi Shao: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
Longsheng Zhao: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
Pingyi Li: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China; Corresponding authors.
Hainan Su: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China; Corresponding authors.
Xiulan Chen: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China
Yuzhong Zhang: State Key Laboratory of Microbial Technology, Marine Biotechnology Research Center, Shandong University, Qingdao 266237, China; College of Marine Life Sciences, Ocean University of China, Qingdao 266237, China; Frontiers Science Center for Deep Ocean Multispheres and Earth System, Ocean University of China, Qingdao 266237, China; Laboratory for Marine Biology and Biotechnology, Pilot National Laboratory for Marine Science and Technology, Qingdao 266237, China

Journal volume & issue: Vol. 2, no. 2
p. 100019

Abstract

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Investigations into the intramolecular interactions of the native protein in solution are important to understand its structural stability as well as its potential uses in future applications. In this study, we used a bacterial two-hybrid system to investigate the interaction between the phycocyanin α and β subunits that form the phycocyanin monomer. Key amino acid residues responsible for the interaction between the subunits were identified, providing direct experimental evidence for the intramolecular interaction.

Published in Engineering Microbiology

ISSN: 2667-3703 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Technology: Chemical technology: Biotechnology; Science: Microbiology
Website: https://www.sciencedirect.com/journal/engineering-microbiology

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