Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers

Zhang Ji; Chuan Zhang; Yang Ye; Yang Ye; Yang Ye; Jiali Ji; Hongguang Dong; Erik Forsberg; Xiaoyu Cheng; Xiaoyu Cheng; Xiaoyu Cheng; Sailing He; Sailing He; Sailing He

doi:10.3389/fbioe.2021.735711

Frontiers in Bioengineering and Biotechnology (Sep 2021)

Magnetically Enhanced Liquid SERS for Ultrasensitive Analysis of Bacterial and SARS-CoV-2 Biomarkers

Zhang Ji,
Chuan Zhang,
Yang Ye,
Yang Ye,
Yang Ye,
Jiali Ji,
Hongguang Dong,
Erik Forsberg,
Xiaoyu Cheng,
Xiaoyu Cheng,
Xiaoyu Cheng,
Sailing He,
Sailing He,
Sailing He

Affiliations

Zhang Ji: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Chuan Zhang: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Yang Ye: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Yang Ye: Ningbo Research Institute, Ningbo, China
Yang Ye: ZJU-TU/e Joint Research Institute of Design, Optoelectronic and Sensing, Hangzhou, China
Jiali Ji: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Hongguang Dong: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Erik Forsberg: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Xiaoyu Cheng: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Xiaoyu Cheng: Ningbo Research Institute, Ningbo, China
Xiaoyu Cheng: ZJU-TU/e Joint Research Institute of Design, Optoelectronic and Sensing, Hangzhou, China
Sailing He: National Engineering Research Center for Optical Instruments, College of Optical Science and Engineering, Zhejiang University, Hangzhou, China
Sailing He: Ningbo Research Institute, Ningbo, China
Sailing He: ZJU-TU/e Joint Research Institute of Design, Optoelectronic and Sensing, Hangzhou, China

DOI: https://doi.org/10.3389/fbioe.2021.735711
Journal volume & issue: Vol. 9

Abstract

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In this work, it is shown that surface-enhanced Raman scattering (SERS) measurements can be performed using liquid platforms to perform bioanalysis at sub-pM concentrations. Using magnetic enrichment with gold-coated magnetic nanoparticles, the high sensitivity was verified with nucleic acid and protein targets. The former was performed with a DNA fragment associated with the bacteria Staphylococcus aureus, and the latter using IgG antibody, a biomarker for COVID-19 screening. It is anticipated that this work will inspire studies on ultrasensitive SERS analyzers suitable for large-scale applications, which is particularly important for in vitro diagnostics and environmental studies.

Published in Frontiers in Bioengineering and Biotechnology

ISSN: 2296-4185 (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Chemical technology: Biotechnology
Website: http://www.frontiersin.org/bioengineering_and_biotechnology

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