Application of Diatoms Quantitative Analysis in the Diagnosis of Drowning

Abstract

ObjectiveTo retrospectively analyze diatom test cases of corpses in water and discuss the value of quantitative analysis of diatoms in the diagnosis of drowning.MethodsA total of 490 cases of water-related death were collected. They were divided into drowning group and postmortem immersion group according to the cause of death. Diatoms in lung, liver, kidney tissue and water sample were analyzed quantitatively by microwave digestion-vacuum filtration-automated scanning electron microscopy （MD-VF-Auto SEM） method. The ratios of content of diatoms in lung tissue and water sample （CL/CD） were calculated.ResultsThe results of diatom test for three organs （lung, liver and kidney） were all positive in 400 cases （85.5%）; the content of diatom in lung, liver, kidney tissues, and water samples of drowning group were （113 235.9±317 868.1）, （26.7±75.6）, （23.3±52.2） and （12 113.3±21 760.0） cells/10 g, respectively; the species of diatom were （7.5±2.8）, （2.6±1.9）, （2.9±2.1） and （8.9±3.0） types, respectively; the CL/CD of drowning group and postmortem immersion group were （100.6±830.7） and （0.3±0.4）, respectively.ConclusionQuantitative analysis of diatoms can provide supportive evidence for the diagnosis of drowning, and the parameter CL/CD can be introduced into the analysis to make a more accurate diagnosis of drowning.

Keywords

• forensic pathology
• drowning
• diatom test
• quantitative analysis
• microwave digestion-vacuum filtration-automated scanning electron microscopy method