Investigation of antioxidant interaction between ascorbic acid and rutin in medicine “Ascorutin”

Abstract

Phytomedicines are combined pharmaceuticals containing various groups of phenolic compounds. It is known that phenolic compounds are powerful antioxidants, but their level of antioxidant activity during interactions has not been previously studied. The aim of the work was to investigate interaction between ascorbic acid and rutin in medicine “Ascorutin”. Materials and methods. The objects of the study were one series of tablets “Ascorutin,” rutin, and ascorbic acid. The level of antioxidant activity was measured by the potentiometric method. Results. The level of antioxidant activity of model solutions of rutin was 265.51 ± 1.00 mmol-eq./L, ascorbic acid – 99.00 ± 1.00 mmol-eq./L, a combination of rutin and ascorbic acid – 255.60 ± 1.00 mmol-eq./L, and medicine “Ascorutin” – 256.52 ± 1.00 mmol-eq./L. An experimental value of the level of antioxidant activity of “Ascorutin”, the combination of rutin and ascorbic acid was 30 % less than theoretical values. Conclusions. The antioxidant interaction of ascorbic acid and rutin in “Ascorutin” medicine has been studied. It has been established that the combination of rutin and ascorbic acid has an antagonistic effect. The findings of this research will serve as a stimulus for exploring the interaction of bioactive substances and selecting their optimal combinations for the development of pharmaceuticals, special food products, food, and cosmetic products.

Keywords

• antioxidant activity
• interaction
• rutin
• ascorbic acid
• antagonism