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BioTechniques
(Dec 2016)
[Letter to the Editor] Many commercial hot-start polymerases demonstrate activity prior to thermal activation
Aaron J. Stevens,
Sarah Appleby,
Martin A. Kennedy
Affiliations
Aaron J. Stevens
1Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand
Sarah Appleby
1Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand
Martin A. Kennedy
1Gene Structure and Function Laboratory, Department of Pathology, University of Otago, Christchurch, New Zealand
DOI
https://doi.org/10.2144/000114481
Journal volume & issue
Vol. 61, no. 6
pp. 293 – 296
Abstract
Read online
No abstracts available.
Keywords
PCR
hot-start polymerase
primer-dimer
polymerase activity
quality control
DNA amplification
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