Scientific Data (Oct 2024)

Chromosome-scale genome assembly of Astragalus membranaceus using PacBio and Hi-C technologies

  • Huijie Fan,
  • Zhi Chai,
  • Xukui Yang,
  • Ake Liu,
  • Haifeng Sun,
  • Zhangyan Wu,
  • Qingshan Li,
  • Cungen Ma,
  • Ran Zhou

DOI
https://doi.org/10.1038/s41597-024-03852-6
Journal volume & issue
Vol. 11, no. 1
pp. 1 – 11

Abstract

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Abstract Astragalus membranaceus (Fisch.) Bge (AM) is a medicinal herb plant belonging to the Leguminosae family. In this study, we present a chromosome-scale genome assembly of AM, aiming to enhance the molecular biology and functional studies of Astragali Radix. The genome size of AM is about 1.43 Gb, with a contig N50 value of 1.67 Mb. A total of 98.16% of the assembly anchored to 9 pseudochromosomes using Hi-C technology. The assembly completeness was estimated to be 97.27% using BUSCO with the long terminal repeat assembly index (LAI) of 16.22 and quality value (QV) of 48.58. Additionally, the genome contained 67.98% repetitive sequences. Genome annotation predicted 29,914 protein-coding genes, including 73 genes involved in the flavonoid biosynthetic pathway and 2,048 transcription factors. The high-quality genome assembly and gene annotation resources will greatly facilitate future functional genomic studies in Leguminosae species.