Silencing Quorum Sensing through Extracts of Melicope lunu-ankenda

Abstract

Quorum sensing regulates bacterial virulence determinants, therefore making it an interesting target to attenuate pathogens. In this work, we screened edible, endemic plants in Malaysia for anti-quorum sensing properties. Extracts from Melicope lunu-ankenda (Gaertn.) T. G. Hartley, a Malay garden salad, inhibited response of Chromobacterium violaceum CV026 to N-hexanoylhomoserine lactone, thus interfering with violacein production; reduced bioluminescence expression of E. coli [pSB401], disrupted pyocyanin synthesis, swarming motility and expression of lecA::lux of Pseudomonas aeruginosa PAO1. Although the chemical nature of the anti-QS compounds from M. lunu-ankenda is currently unknown, this study proves that endemic Malaysian plants could serve as leads in the search for anti-quorum sensing compounds.

Keywords

• anti-quorum sensing
• bioluminescence
• lecA::lux
• Malaysian plants
• N-acyl-L-homoserine lactones (AHL)
• Pseudomonas aeruginosa PAO1
• pyocyanin
• virulence
• swarming motility