A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Liu Jian; Qian Weidong; Wang Jian; Bai Yilan; Gui Yaping; Xia Luming; Gong Guohua; Ge Feifei; Shen Haixiao; Chang Xiaojing; Zhao Hongjin

doi:10.33073/pjm-2023-029

Polish Journal of Microbiology (Sep 2023)

A Recombinase-Aided Amplification Assay for the Detection of Chlamydia felis

Liu Jian,
Qian Weidong,
Wang Jian,
Bai Yilan,
Gui Yaping,
Xia Luming,
Gong Guohua,
Ge Feifei,
Shen Haixiao,
Chang Xiaojing,
Zhao Hongjin

Affiliations

Liu Jian: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Qian Weidong: School of Food and Biological Engineering, Shaanxi University of Science and Technology, Xi’an, China
Wang Jian: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Bai Yilan: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Gui Yaping: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Xia Luming: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Gong Guohua: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Ge Feifei: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Shen Haixiao: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Chang Xiaojing: Shanghai Animal Disease Prevention and Control Center, Shanghai, China
Zhao Hongjin: Shanghai Animal Disease Prevention and Control Center, Shanghai, China

DOI: https://doi.org/10.33073/pjm-2023-029
Journal volume & issue: Vol. 72, no. 3
pp. 339 – 343

Abstract

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Chlamydia felis is an important zoonotic agent for humans and various animals. A recombinase-aided amplification (RAA) assay was developed for detecting C. felis. RAA can be performed in a closed tube at 39°C within 30 min. The detection limit was 10.6 copies of the C. felis plasmid DNA per reaction. No positive signals for other pathogens were detected. The coincidence rate of RAA and conventional PCR was 95.24% (20/21) and 100% (96/96) for positive and negative samples, respectively. The established RAA assay is a simple, rapid, highly sensitive, and specific method for detecting C. felis.

Published in Polish Journal of Microbiology

ISSN: 1733-1331 (Print); 2544-4646 (Online)
Publisher: Sciendo
Country of publisher: Poland
LCC subjects: Science: Biology (General): Genetics; Science: Microbiology
Website: https://www.sciendo.com/journal/PJM

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