Frontiers in Bioengineering and Biotechnology (Oct 2023)

Advancements in the synergy of isothermal amplification and CRISPR-cas technologies for pathogen detection

  • Xiaolei Mao,
  • Minghui Xu,
  • Shuyin Luo,
  • Yi Yang,
  • Jiaye Zhong,
  • Jiawei Zhou,
  • Huayan Fan,
  • Xiaoping Li,
  • Xiaoping Li,
  • Zhi Chen

DOI
https://doi.org/10.3389/fbioe.2023.1273988
Journal volume & issue
Vol. 11

Abstract

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In the realm of pathogen detection, isothermal amplification technology has emerged as a swift, precise, and sensitive alternative to conventional PCR. This paper explores the fundamental principles of recombinase polymerase amplification (RPA) and recombinase-aid amplification (RAA) and reviews the current status of integrating the CRISPR-Cas system with RPA/RAA techniques. Furthermore, this paper explores the confluence of isothermal amplification and CRISPR-Cas technology, providing a comprehensive review and enhancements of existing combined methodologies such as SHERLOCK and DETECTR. We investigate the practical applications of RPA/RAA in conjunction with CRISPR-Cas for pathogen detection, highlighting how this integrated approach significantly advances both research and clinical implementation in the field. This paper aims to provide readers with a concise understanding of the fusion of RPA/RAA and CRISPR-Cas technology, offering insights into their clinical utility, ongoing enhancements, and the promising prospects of this integrated approach in pathogen detection.

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