RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Joseph Abatemarco; Maen F. Sarhan; James M. Wagner; Jyun-Liang Lin; Leqian Liu; Wafa Hassouneh; Shuo-Fu Yuan; Hal S. Alper; Adam R. Abate

doi:10.1038/s41467-017-00425-7

Nature Communications (Aug 2017)

RNA-aptamers-in-droplets (RAPID) high-throughput screening for secretory phenotypes

Joseph Abatemarco,
Maen F. Sarhan,
James M. Wagner,
Jyun-Liang Lin,
Leqian Liu,
Wafa Hassouneh,
Shuo-Fu Yuan,
Hal S. Alper,
Adam R. Abate

Affiliations

Joseph Abatemarco: Department of Chemical Engineering, The University of Texas at Austin
Maen F. Sarhan: Bioengineering and Therapeutic Sciences, University of California San Francisco
James M. Wagner: Department of Chemical Engineering, The University of Texas at Austin
Jyun-Liang Lin: Department of Chemical Engineering, The University of Texas at Austin
Leqian Liu: Bioengineering and Therapeutic Sciences, University of California San Francisco
Wafa Hassouneh: Bioengineering and Therapeutic Sciences, University of California San Francisco
Shuo-Fu Yuan: Institute for Cellular and Molecular Biology, The University of Texas at Austin
Hal S. Alper: Department of Chemical Engineering, The University of Texas at Austin
Adam R. Abate: Bioengineering and Therapeutic Sciences, University of California San Francisco

DOI: https://doi.org/10.1038/s41467-017-00425-7
Journal volume & issue: Vol. 8, no. 1
pp. 1 – 9

Abstract

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Screening libraries of genetically engineered microbes for secreted products is limited by the available assay throughput. Here the authors combine aptamer-based fluorescent detection with droplet microfluidics to achieve high throughput screening of yeast strains engineered for enhanced tyrosine or streptavidin production.

Published in Nature Communications

ISSN: 2041-1723 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science
Website: https://www.nature.com/ncomms/

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