RUHS Journal of Health Sciences (Oct 2023)

Detection and Assessment of Human Cytomegalo Virus, Epstein -Barr Virus -1 and Herpes Simplex Virus in Patients with Chronic Periodontitis of Varying Pocket Depths

  • Charu Agrawal; Pushpa Pudakalakatti; Mishal P Shah; Kishore G Bhat; Shilpa Patil;, Shikha Gupta

DOI
https://doi.org/10.37821/ruhsjhs.2.4.2017.174-180

Abstract

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Introduction: Recent microbiological research suggests an important role of herpes viruses in the pathogenesis of periodontal disease. Understanding the role of viruses in periodontal disease will lead to better prevention and treatment of the disease. Thus, the present study was undertaken to detect and assess the presence of human cytomegalovirus (HCMV), Epstein -Barr virus -1 (EBV-1) and herpes simplex virus (HST) in chronic periodontitis patients and also to determine the correlation between clinical parameters and presence of herpes viruses. Methodology: 30 patients with chronic periodontitis participated in the study. Subgingival plaque samples were obtained from one deep and one shallow site of each patient. DNA extractions were done from these samples and polymerase chain reaction (PCR) assay was carried out to detect the viral DNA. Statistical analysis of the results was done using Z - test of proportion. Results: The detection frequency of HSV-1 was found to be highest in deep (73.33%) as well as shallow (53.33%) sites among all the viruses followed by EBV-1, HSV-2 and HCMV. The detection frequency of all the four viruses was found to be higher in deep sites as compared to shallow sites and the results were found to be statistically highly significant in case of EBV-1 and HSV-2. Statistically highly significant differences were found when comparisons were made between plaque index and presence of viruses in deep (both viral detected and undetected) sites but not in relation to shallow sites. There were no statistically significant differences between gingival index and the presence of viruses in both deep as well as shallow sites, except for HCMV, where the results were found to be significant. Conclusion: Detection frequency of herpes viruses mainly HSV-1 and EBV-1 was higher in deep sites as compared to shallow sites.

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