Nature Communications (Apr 2019)
Development and application of a high-content virion display human GPCR array
- Guan-Da Syu,
- Shih-Chin Wang,
- Guangzhong Ma,
- Shuang Liu,
- Donna Pearce,
- Atish Prakash,
- Brandon Henson,
- Lien-Chun Weng,
- Devlina Ghosh,
- Pedro Ramos,
- Daniel Eichinger,
- Ignacio Pino,
- Xinzhong Dong,
- Jie Xiao,
- Shaopeng Wang,
- Nongjian Tao,
- Kwang Sik Kim,
- Prashant J. Desai,
- Heng Zhu
Affiliations
- Guan-Da Syu
- Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine
- Shih-Chin Wang
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Guangzhong Ma
- Biodesign Center for Bioelectronics and Biosensors, Arizona State University
- Shuang Liu
- Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine
- Donna Pearce
- Division of Paediatric Infectious Diseases, Johns Hopkins University School of Medicine
- Atish Prakash
- Division of Paediatric Infectious Diseases, Johns Hopkins University School of Medicine
- Brandon Henson
- Viral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Lien-Chun Weng
- Viral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Devlina Ghosh
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Pedro Ramos
- CDI Laboratories, Inc.
- Daniel Eichinger
- CDI Laboratories, Inc.
- Ignacio Pino
- CDI Laboratories, Inc.
- Xinzhong Dong
- Solomon H. Snyder Department of Neuroscience, Johns Hopkins University School of Medicine
- Jie Xiao
- Department of Biophysics and Biophysical Chemistry, Johns Hopkins University School of Medicine
- Shaopeng Wang
- Biodesign Center for Bioelectronics and Biosensors, Arizona State University
- Nongjian Tao
- Biodesign Center for Bioelectronics and Biosensors, Arizona State University
- Kwang Sik Kim
- Division of Paediatric Infectious Diseases, Johns Hopkins University School of Medicine
- Prashant J. Desai
- Viral Oncology Program, Department of Oncology, The Sidney Kimmel Comprehensive Cancer Center, Johns Hopkins University School of Medicine
- Heng Zhu
- Department of Pharmacology and Molecular Sciences, Johns Hopkins University School of Medicine
- DOI
- https://doi.org/10.1038/s41467-019-09938-9
- Journal volume & issue
-
Vol. 10,
no. 1
pp. 1 – 12
Abstract
G protein-coupled receptors (GPCRs) are important targets for drug discovery. Here, the authors develop a Virion Display array of 315 functional non-odorant GPCRs, providing a platform for high-throughput, unbiased screening for small molecule drugs, affinity reagents, and microbial interactions.
