Journal of Lipid Research (Dec 1989)

A fast atom bombardment-mass spectrometric method to quantitate lysophosphatidylserine in rat brain.

  • E Benfenati,
  • G De Bellis,
  • S Chen,
  • L Bettazzoli,
  • R Fanelli,
  • M T Tacconi,
  • G Kirschner,
  • G Pege

Journal volume & issue
Vol. 30, no. 12
pp. 1983 – 1986

Abstract

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A method to quantitate lysophosphatidylserine by fast atom bombardment-mass spectrometry using 1-hexa-decanoyl-sn-glycero-3-phospho-L-serine as internal standard is described. The standard curve is linear with a correlation coefficient r2 = 0.999 from 10 to 1000 ng. This curve has been used to quantitate LPS in rat brain using phosphorus assay as a test control. We found 475 +/- 70 ng of LPS in 1 mg of tissue (n = 3). This method presents advantages due to its sensitivity and its capability to give molecular information of the unmodified compound.