Improved assay of 3-hydroxy-3-methylglutaryl coenzyme A reductase

Abstract

Two improvements are described for the assay of HMG CoA reductase. These are a simple synthesis of the substrate precursor HMG-3-14C anhydride and a double-label (14C and 3H) method for determining the amount of mevalonate-3-14C that is formed from the substrate.

Keywords

• 3-hydroxy-3-methylglutaric anhydride
• double-label assay
• thin-layer chromatography
• cholesterol biosynthesis