Bioactive Components From Gracilaria rubra With Growth Inhibition on HCT116 Colon Cancer Cells and Anti-inflammatory Capacity in RAW 264.7 Macrophages

Lingxiao Yi; Qi Wang; Haiyan Luo; Daqing Lei; Zhonghai Tang; Sijia Lei; Hang Xiao

doi:10.3389/fnut.2022.856282

Frontiers in Nutrition (Apr 2022)

Bioactive Components From Gracilaria rubra With Growth Inhibition on HCT116 Colon Cancer Cells and Anti-inflammatory Capacity in RAW 264.7 Macrophages

Lingxiao Yi,
Qi Wang,
Haiyan Luo,
Daqing Lei,
Zhonghai Tang,
Sijia Lei,
Hang Xiao

Affiliations

Lingxiao Yi: Department of Food Science, University of Massachusetts, Amherst, MA, United States
Qi Wang: Department of Food Science, University of Massachusetts, Amherst, MA, United States
Haiyan Luo: Department of Food Science, University of Massachusetts, Amherst, MA, United States
Daqing Lei: School of Food and Drug, Shenzhen Polytechnic, Shenzhen, China
Zhonghai Tang: College of Food Science and Technology, Hunan Agricultural University, Changsha, China
Sijia Lei: School of Food and Drug, Shenzhen Polytechnic, Shenzhen, China
Hang Xiao: Department of Food Science, University of Massachusetts, Amherst, MA, United States

DOI: https://doi.org/10.3389/fnut.2022.856282
Journal volume & issue: Vol. 9

Abstract

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Gracilaria rubra is rich in bioactive compounds with various potential health benefits. This study aimed to elucidate the profile of both extractable bioactive components (EBCs) and non-extractable bioactive components (NEBCs) of G. rubra and determine their anti-colon cancer and anti-inflammatory activities. Both EBCs and NEBCs displayed strong suppressive effects on the viability of HCT116 cells, which causes cell cycle arrest, induces cellular apoptosis, and regulates the expression of cyclin-dependent kinases (CDKs) and tumor suppressor proteins. Additionally, EBCs and NEBCs from G. rubra displayed anti-inflammatory functions via inhibiting the production of nitric oxide (NO), reactive oxygen species (ROS), and proinflammatory cytokines in activated macrophages and regulating the expression levels of cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), NADPH-quinone oxidoreductase-1 (NQO-1), and heme oxygenase 1 (HO-1). These findings provide a rationale for animal and human studies designed to evaluate the chemopreventive and anti-inflammatory potential of these bioactive compounds from G. rubra.

Published in Frontiers in Nutrition

ISSN: 2296-861X (Online)
Publisher: Frontiers Media S.A.
Country of publisher: Switzerland
LCC subjects: Technology: Home economics: Nutrition. Foods and food supply
Website: https://www.frontiersin.org/journals/nutrition/

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