Biomathematical approach determination of a rational formula of complex phytocompositions

Abstract

Despite the huge achievements of pharmaceutical chemistry, herbal medicines based on medicinal plant raw materials (MPRMs) are still in demand for the treatment of both humans and animals diseases. Their main advantage is the small number of contraindications and side effects. In this case, not one but several phytocompositions are often used, which makes it possible to enhance the effectiveness of the drugs and expand the spectrum of action. The key point in creating multicomponent (galenic) preparations is the development of an accurate phytocomposition formula that best meets the set goals. This process is quite complex and timeconsuming. The purpose of this study was to determine the rational formula of complex phytocompositions based on a biomathematical approach. To reduce the number of performed experiments compared to blind enumeration and increase the reliability and efficiency of the quantitative formula of the phytocomposition, we tested a combination of the approach used in mathematics to search for the extremum of functions of many variables with a biological experiment on Parametium caudatum. In this case, the variables were the shares of each MPRM in the final recipe; function was understood as the pharmacological activity of a substance, determined through its membrane-stabilizing and antioxidant effect. This made it possible to determine the effective ratio of phytocomposition components faster and at lower costs. A comparison was made among more than 100 model samples. It has been established that all of them are non-toxic and have a good antioxidant and membranestabilizing effect. The final combination is by 10-20% more effective than other model samples and therefore it is advisable to use it in the future as the main ingredient for obtaining liposomal preparations for external use with a good pharmacological effect.