Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Mei-Hwa Lee; Jeng-Shiung Jan; James L. Thomas; Yuan-Pin Shih; Jin-An Li; Chien-Yu Lin; Tooru Ooya; Lilla Barna; Mária Mészáros; András Harazin; Gergő Porkoláb; Szilvia Veszelka; Maria A. Deli; Hung-Yin Lin

doi:10.3390/cells11162584

Cells (Aug 2022)

Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model

Mei-Hwa Lee,
Jeng-Shiung Jan,
James L. Thomas,
Yuan-Pin Shih,
Jin-An Li,
Chien-Yu Lin,
Tooru Ooya,
Lilla Barna,
Mária Mészáros,
András Harazin,
Gergő Porkoláb,
Szilvia Veszelka,
Maria A. Deli,
Hung-Yin Lin

Affiliations

Mei-Hwa Lee: Department of Materials Science and Engineering, I-Shou University, Kaohsiung 84001, Taiwan
Jeng-Shiung Jan: Department of Chemical Engineering, National Cheng Kung University, Tainan 70101, Taiwan
James L. Thomas: Department of Physics and Astronomy, University of New Mexico, Albuquerque, NM 87131, USA
Yuan-Pin Shih: Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
Jin-An Li: Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
Chien-Yu Lin: Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan
Tooru Ooya: Graduate School of Engineering, Department of Chemical Science and Engineering, Kobe University, Kobe 657-8501, Japan
Lilla Barna: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
Mária Mészáros: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
András Harazin: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
Gergő Porkoláb: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
Szilvia Veszelka: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
Maria A. Deli: Institute of Biophysics, Biological Research Centre, H-6726 Szeged, Hungary
Hung-Yin Lin: Department of Chemical and Materials Engineering, National University of Kaohsiung, Kaohsiung 81148, Taiwan

DOI: https://doi.org/10.3390/cells11162584
Journal volume & issue: Vol. 11, no. 16
p. 2584

Abstract

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Several degenerative disorders of the central nervous system, including Parkinson’s disease (PD), are related to the pathological aggregation of proteins. Antibodies against toxic disease proteins, such as α-synuclein (SNCA), are therefore being developed as possible therapeutics. In this work, one peptide (YVGSKTKEGVVHGVA) from SNCA was used as the epitope to construct magnetic molecularly imprinted composite nanoparticles (MMIPs). These composite nanoparticles were characterized by dynamic light scattering (DLS), high-performance liquid chromatography (HPLC), isothermal titration calorimetry (ITC), Brunauer–Emmett–Teller (BET) analysis, and superconducting quantum interference device (SQUID) analysis. Finally, the viability of brain endothelial cells that were treated with MMIPs was measured, and the extraction of SNCA from CRISPR/dCas9a-activated HEK293T cells from the in vitro model system was demonstrated for the therapeutic application of MMIPs.

Published in Cells

ISSN: 2073-4409 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Science: Biology (General): Cytology
Website: https://www.mdpi.com/journal/cells

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