Pengaruh Propylthiuouracil terhadap Kadar Testosteron dan Estrogen pada Tikus Sprague Dawley Betina

Abstract

ABSTRACT Iodine Deficiency Disorders (IDD) was associated with female reproductive hormones disorders. Hypothyroidism inhibits follicular development which subsequently inhibited estrogen secretion. Biosynthesis of estrogen derived from testosterone through aromatization mechanism in ovarian granulose cells. This study aimed to determine changes in levels of serum testosterone and estrogen in hypothyroid rats induced by propylthiouracil. This study was an experimental post test only with controlled group design. Study used 11 Sprague dawley female rats aged 2.5 months with an average body weight 111.1 grams. Five rats given propylthiouracil in the drinking water with doses of 0.1 g/L for 30 days and six rats were used as controls. Hypothyroid rats were determined by measurements of free T4 levels. Data of free T4 levels, testosterone, and estrogen taken from blood serum and it were measured using ELISA technique. Data of testosterone, estrogen, and free T4 analysis using independent sample t test. Free T4 levels of treatment group were 4.0 ± 0.4 pmol/L and the controlled group were 8.1 ± 1.6 pmol/L, statistical analysis showed significant difference between the two groups with p values = 0.001. Testosterone levels of the treatment group were 0.72 ± 0.1 pg/dL and control group were 0.66 ± 0.1 pg/dL, statistical analysis showed no significant difference between the two groups with p values = 0.20. Estrogen levels of the treatment group were 9.4 ± 6.2 pg/dL and control group were 12.6 ± 3.9 pg/dL, statistical analysis showed no significant difference between the two groups with p values = 0.35. There was no significant change in the levels of testosterone and estrogen serum in Sprague dawley female hypothyroid rats induced by propylthiouracil. Keywords: hypothyroidism, testosterone, estrogen, free T4, Sprague dawley. ABSTRAK Gangguan Akibat Kekurangan Iodium (GAKI) ada hubungannya dengan hormon reproduksi perempuan. Hipotiroid menghambat perkembangan folikuler sehingga sekresi estrogen terhambat. Biosintesis hormon estrogen berasal dari hormon testosteron melalui mekanisme aromatisasi di dalam sel granulosa ovarium. Penelitian ini bertujuan mengetahui perubahan kadar hormon testosteron dan estrogen serum pada tikus hipotiroid dengan induksi propylthiouracil. Penelitian ini adalah studi eksperimental dengan rancangan post test only with controlled group, menggunakan 11 tikus jenis Sprague dawley betina berumur 2,5 bulan dengan berat badan rata-rata 111,1 gram. Lima ekor tikus sebagai perlakuan diberi propylthiouracil dalam air minum dengan kadar 0,1 g/L selama 30 hari dan enam ekor tikus digunakan sebagai kontrol. Untuk menentukan tikus hipotiroid digunakan pengukuran kadar T4 bebas. Data kadar T4 bebas, testosteron, dan estrogen diambil dari serum darah dan diukur dengan menggunakan teknik ELISA. Data kadar testosteron, estrogen, dan T4 bebas diuji dengan menggunakan uji statistik t tidak berpasangan. Kadar T4 bebas kelompok perlakuan (4,0 ± 0,4 pmol/L) dan kelompok kontrol (8,1 ± 1,6 pmol/L), uji statistik menunjukkan beda bermakna antara kedua kelompok dengan nilai p = 0,001. Kadar testosteron pada kelompok perlakuan (0,72 ± 0,1 pg/dL) dan kelompok kontrol (0,66 ± 0,1 pg/dL), uji statistik menunjukkan tidak beda bermakna antara kedua kelompok dengan nilai p = 0,20. Kadar estrogen pada kelompok perlakuan (9,1 ± 6,2 pg/dL) dan kelompok kontrol (12,6 ± 3,9 pg/dL), analisis statistik menunjukkan tidak beda bermakna antara kedua kelompok dengan nilai p = 0,35. Tidak terjadi perubahan signifikan kadar testosteron dan estrogen serum pada tikus Sprague dawley betina hipotiroid dengan induksi propylthiouracil. Kata kunci: hipotiroid, testosteron, estrogen, T4 bebas, Sprague dawley.