Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae

Sagar Raturi; Asha V. Nair; Keiko Shinoda; Himansha Singh; Boyan Bai; Satoshi Murakami; Hideaki Fujitani; Hendrik W. van Veen

doi:10.1038/s42003-021-02081-6

Communications Biology (May 2021)

Engineered MATE multidrug transporters reveal two functionally distinct ion-coupling pathways in NorM from Vibrio cholerae

Sagar Raturi,
Asha V. Nair,
Keiko Shinoda,
Himansha Singh,
Boyan Bai,
Satoshi Murakami,
Hideaki Fujitani,
Hendrik W. van Veen

Affiliations

Sagar Raturi: Department of Pharmacology, University of Cambridge
Asha V. Nair: Department of Pharmacology, University of Cambridge
Keiko Shinoda: Microbial Membrane Transport Engineering, Biotechnology Research Center, The University of Tokyo
Himansha Singh: Department of Pharmacology, University of Cambridge
Boyan Bai: Department of Pharmacology, University of Cambridge
Satoshi Murakami: Department of Life Science and Technology, Tokyo Institute of Technology
Hideaki Fujitani: Laboratories for Systems Biology and Medicine, Research Center for Advanced Science and Technology, The University of Tokyo
Hendrik W. van Veen: Department of Pharmacology, University of Cambridge

DOI: https://doi.org/10.1038/s42003-021-02081-6
Journal volume & issue: Vol. 4, no. 1
pp. 1 – 17

Abstract

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Raturi and Nair et al. describe functional ion pathways within the MATE transporter NorM from Vibrio cholerae (NorM-VC). By creating chimeric proteins and mutants of NorM-VC and NorM-PS, they dissect the localisation of ion-coupling events and roles of conserved catalytic carboxylates in H+ binding and Na+ coordination, with findings useful to the field of transporters and drug resistance pumps.

Published in Communications Biology

ISSN: 2399-3642 (Online)
Publisher: Nature Portfolio
Country of publisher: United Kingdom
LCC subjects: Science: Biology (General)
Website: https://www.nature.com/commsbio/

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