Post-replicative nick translation occurs on the lagging strand during prolonged depletion of DNA ligase I in <i>Saccharomyces cerevisiae</i>

Abstract

AbstractDuring lagging-strand synthesis, strand-displacement synthesis by DNA polymerase delta (Pol ∂), coupled to nucleolytic cleavage of DNA flap structures, produces a nick-translation reaction that replaces the DNA at the 5′ end of the preceding Okazaki fragment. Previous work following depletion of DNA ligase I in Saccharomyces cerevisaein vivoSaccharomyces cerevisiaein vivo