Біологія тварин (May 2020)
Hematological indices of rats after administration of enrofloxacin as a subunit of polymer
Abstract
The influence of antibiotic enrofloxacin and PEG-400 polymer complex on hemoglobin content, red and white blood cell count, and blood leukogram state of apparently healthy rats has been studied. The enrofloxacin contains reactive carboxyl groups in the molecule structure, it makes possible to carry out modifications for obtaining new compounds. The complex of enrofloxacin with polymer was obtained by interaction of enrofloxacin hydrochloride with polyethylene glycol-400. There were not any abnormal changes in the physiological state of the animals after administration none of the tested substances. The effect of the enrofloxacin + PEG400 complex in 7 days after the injection was manifested by the decrease of red blood cells count and hemoglobin concentration in the rats’ blood, and after 14 and 21 days — by activation of hematopoietic function. Particularly, on the 14th day red blood cell counts in the blood of control animals and animals treated with enrofloxacin+PEG-400 complex were approximately equal and ranged 4.6–4.7×1012/L, and were 8.0–16.4% (P>0.05) lower than in experimental rats received the antibiotic and PEG-400 separately. At the same time, in the absence of significant difference in red blood cell count, the blood hemoglobin concentration increased. This concentration reached a maximum after the administration of the enrofloxacin + PEG-400 complex (132.2±4.10 g/L), but it became a little bit lower (2.7–8.9 g/L) in rats’ blood of other experimental groups. The effects of PEG-400 and enrofloxacin both separately and in complex of enrofloxacin + PEG400, were characterized by the decrease in leukocytes count throughout the experiment. So, after the 21st day of experiment white blood cell count was the lowest in the blood of animals after the enrofloxacin + PEG-400 complex administration (4.2±0.41×109/L; P<0.001) in comparison with the control group; but when the enrofloxacin and PEG-400 were administered separately, white blood cell count was 14.5 (P<0.05) and 34.8% (P<0.01) lower, respectively. Changes in the ratio of different types of leukocytes in the blood of rats characterize the reaction of the body to the introduction of the test substances, the intensity of which decays on the 7th day of the experiment.
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