Generation of an induced pluripotent stem cell (iPSC) line (BBANTWi009-A) from a Meester-Loeys syndrome patient carrying a BGN mutation

Pauline De Kinderen; Laura Rabaut; Anne Hebert; Peter Ponsaerts; Melanie Perik; Josephina A.N. Meester

Stem Cell Research (Feb 2023)

Generation of an induced pluripotent stem cell (iPSC) line (BBANTWi009-A) from a Meester-Loeys syndrome patient carrying a BGN mutation

Pauline De Kinderen,
Laura Rabaut,
Anne Hebert,
Peter Ponsaerts,
Melanie Perik,
Josephina A.N. Meester

Affiliations

Pauline De Kinderen: Center of Medical Genetics, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium
Laura Rabaut: Center of Medical Genetics, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium
Anne Hebert: Center of Medical Genetics, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium
Peter Ponsaerts: Laboratory of Experimental Hematology, Vaccine and Infectious Disease Institute (Vaxinfectio), University of Antwerp, Antwerp, Belgium
Melanie Perik: Center of Medical Genetics, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium
Josephina A.N. Meester: Center of Medical Genetics, University of Antwerp and Antwerp University Hospital, Antwerp, Belgium; Corresponding author at: Cardiogenetics, Center of Medical Genetics, University of Antwerp/Antwerp University Hospital, Antwerp, Belgium.

Journal volume & issue: Vol. 66
p. 103009

Abstract

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Meester-Loeys syndrome (MRLS) is an X-linked syndromic form of thoracic aortic aneurysm and dissection. Here, we report an iPSC line (BBANTWi009-A) of a boy carrying a hemizygous BGN mutation (chrX:153502980-153530518del, GRCh38) causing MRLS. iPSCs were generated from dermal fibroblasts by reprogramming with the Cytotune®-iPS 2.0 Sendai Reprogramming Kit (Invitrogen). The generated iPSCs showed a normal karyotype, expressed pluripotency markers, were differentiated into the three germ layers and carried the original genotype.

Published in Stem Cell Research

ISSN: 1873-5061 (Print); 1876-7753 (Online)
Publisher: Elsevier
Country of publisher: Netherlands
LCC subjects: Science: Biology (General)
Website: https://www.journals.elsevier.com/stem-cell-research

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