Untargeted Metabolomics Used to Describe the Chemical Composition, Antioxidant and Antimicrobial Effects of Extracts from <i>Pleurotus</i> spp. Mycelium Grown in Different Culture Media

Giancarlo Angeles Flores; Carolina Elena Girometta; Gaia Cusumano; Paola Angelini; Bruno Tirillini; Federica Ianni; Francesca Blasi; Lina Cossignani; Roberto Maria Pellegrino; Carla Emiliani; Roberto Venanzoni; Giuseppe Venturella; Pasqualina Colasuonno; Fortunato Cirlincione; Maria Letizia Gargano; Gokhan Zengin; Alessandra Acquaviva; Simonetta Cristina Di Simone; Giustino Orlando; Luigi Menghini; Claudio Ferrante

doi:10.3390/antibiotics11111468

Antibiotics (Oct 2022)

Untargeted Metabolomics Used to Describe the Chemical Composition, Antioxidant and Antimicrobial Effects of Extracts from <i>Pleurotus</i> spp. Mycelium Grown in Different Culture Media

Giancarlo Angeles Flores,
Carolina Elena Girometta,
Gaia Cusumano,
Paola Angelini,
Bruno Tirillini,
Federica Ianni,
Francesca Blasi,
Lina Cossignani,
Roberto Maria Pellegrino,
Carla Emiliani,
Roberto Venanzoni,
Giuseppe Venturella,
Pasqualina Colasuonno,
Fortunato Cirlincione,
Maria Letizia Gargano,
Gokhan Zengin,
Alessandra Acquaviva,
Simonetta Cristina Di Simone,
Giustino Orlando,
Luigi Menghini,
Claudio Ferrante

Affiliations

Giancarlo Angeles Flores: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Carolina Elena Girometta: Department of Earth and Environmental Sciences (DSTA), University of Pavia, 27100 Pavia, Italy
Gaia Cusumano: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Paola Angelini: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Bruno Tirillini: Department of Biomolecular Sciences, University of Urbino, 61029 Urbino, Italy
Federica Ianni: Department of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, Italy
Francesca Blasi: Department of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, Italy
Lina Cossignani: Department of Pharmaceutical Sciences, University of Perugia, 06126 Perugia, Italy
Roberto Maria Pellegrino: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Carla Emiliani: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Roberto Venanzoni: Department of Chemistry, Biology and Biotechnology, University of Perugia, 06122 Perugia, Italy
Giuseppe Venturella: Department of Agricultural, Food and Forest Sciences, University of Palermo, Viale delle Scienze, Bldg. 5, 90128 Palermo, Italy
Pasqualina Colasuonno: Department of Agricultural, Food and Forest Sciences, University of Palermo, Viale delle Scienze, Bldg. 5, 90128 Palermo, Italy
Fortunato Cirlincione: Department of Agricultural, Food and Forest Sciences, University of Palermo, Viale delle Scienze, Bldg. 5, 90128 Palermo, Italy
Maria Letizia Gargano: Department of Agricultural and Environmental Science, University of Bari Aldo Moro, Via Amendola 165/A, 70126 Bari, Italy
Gokhan Zengin: Physiology and Biochemistry Research Laboratory, Department of Biology, Science Faculty, Selcuk University, 42130 Konya, Turkey
Alessandra Acquaviva: Botanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, Italy
Simonetta Cristina Di Simone: Botanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, Italy
Giustino Orlando: Botanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, Italy
Luigi Menghini: Botanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, Italy
Claudio Ferrante: Botanic Garden “Giardino dei Semplici”, Department of Pharmacy, “Gabriele d’Annunzio” University, Via dei Vestini 31, 66100 Chieti, Italy

DOI: https://doi.org/10.3390/antibiotics11111468
Journal volume & issue: Vol. 11, no. 11
p. 1468

Abstract

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Pleurotus species isolated in vitro were studied to determine the effect of different media on their production of secondary metabolites, antimicrobial, and antioxidant activity. The different metabolites among Pleurotus samples covered a total of 58 pathways. Comparisons were made between the metabolic profiles of Pleurotus spp. mycelia grown in two substrates: Potato-dextrose-agar-PDA, used as control (S1), and PDA enriched with 0.5 % of wheat straw (S2). The main finding was that the metabolic pathways are strongly influenced by the chemical composition of the growth substrate. The antibacterial effects were particularly evident against Escherichia coli, whereas Arthroderma curreyi (CCF 5207) and Trichophyton rubrum (CCF 4933) were the dermatophytes more sensitive to the mushroom extracts. The present study supports more in-depth investigations, aimed at evaluating the influence of growth substrate on Pleurotus spp. antimicrobial and antioxidant properties.

Published in Antibiotics

ISSN: 2079-6382 (Online)
Publisher: MDPI AG
Country of publisher: Switzerland
LCC subjects: Medicine: Therapeutics. Pharmacology
Website: http://www.mdpi.com/journal/antibiotics

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