Genotypic diversity and abiotic stress response profiling of short-grain aromatic landraces of rice (Oryza sativa L. Indica)

Abstract

This study evaluated the physiological responses and genetic diversity of selected short-grain aromatic rice landraces of Northern Eastern Ghats of India to multiple abiotic stresses. Results revealed that drought, salt and flooding stress remarkably declined plant biomass, relative growth index (RGI), relative water content (RWC), leaf photosynthesis, PSII activity, SPAD relative index, and elevated effect was found in susceptible IR64 (susceptible check) variety. We employed molecular marker analysis to characterize 21 rice genotypes using 32 SSR primers related to drought, flooding and salinity tolerance QTLs. Our analysis suggests significantly high polymorphism (93.12%) in 96 loci. The mean value of polymorphism information content (PIC), marker index (MI) and resolving power (RP) and heterozygosity index (HI) were 0.326, 1.037, 1.558 and 0.420 respectively. The Jaccard’s similarity coefficient ranged from 0.158 to 0.846 with a mean genetic dissimilarity of 0.647. According to neighbor-joining method of clustering, all genotypes were grouped into two major and five sub-clusters which is concurrent with a very broad genetic base (K = 5) obtained with STRUCTURE analysis. Taken together, six aromatic genotypes Basubhoga, Dudhamani, Kalajeera, Laktimachi, Kuyerkuling and Tulasi are genetically very close to drought-tolerant (N22) and flooding tolerant (FR13A) cultivar whereas, salt-tolerant (Pokkali) very close to Sapuri and Muktabali. The degree of genetic variants obtained from this profiling could be useful for landrace conservation, selection and global implications for multiple abiotic stress tolerance.

Keywords

• Allelic diversity
• Multiple abiotic stress
• Photosynthesis
• Traditional rice
• STRUCTURE analysis