Genetic and phenotypic relationships among Pinus sylvestris populations in the Pieniny National Park

Abstract

The phenotypic and genetic relationships among five Pinus sylvestris populations in the western part of the Carpathian Mountains were analyzed based on seven morphological needle traits and 67 loci using 10 RAPD primers. Three of the studied populations were on the western side (Macelowa Gora, Czubatka, Łazowa Skałka) and two were located in the central part (Sokolica, Zamkowa Gora) of the Pieniny National Park. The examined populations had short needles, which is characteristic for mountain habitats of the Scots pine. The populations from the Pieniny Mts. had fewer rows of stomata on both sides of the needles than others studied stands of Eurosiberian Scots pine. The number of stomata on both flat and convex sides of the needles was similar to the values observed for populations growing on the nearby Tatra Mountains. Moreover, when these two traits are considered, the populations from Sokolica and Łazowa Skałka share the highest degree of similarity.. Nei’s genetic distances and genetic identities between the populations were calculated on the basis of RAPD loci. The results show that the lowest Nei’s distance exists between the populations from the western part of the Pieniny Mts. – Macelowa Gora and Czubatka, and its central part – Sokolica and Zamkowa Gora, with the highest values recorded between the populations of Zamkowa Gora and Czubatka, and Łazowa Skałka and Czubatka. A similar pattern of phenotypic and genetic relations that was observed between the populations in both regions of the Pieniny Mountains could be the result of planting-seed material sourced from the central Pieniny Mts. in the area of western Pieniny Mts.

Keywords

• Scots pine
• Western Carpathians
• RAPD
• pine needle morphology
• population differentiation