5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma

Chunyu Zhang; Wei Zhou; Yinqiu Tan; Daofeng Tian; Chunlong Zhong

Heliyon (Oct 2022)

5-Hydroxymethylcytosines in circulating cell-free DNA reveal a diagnostic biomarker for glioma

Chunyu Zhang,
Wei Zhou,
Yinqiu Tan,
Daofeng Tian,
Chunlong Zhong

Affiliations

Chunyu Zhang: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Department of Neurosurgery, Huzhou Central Hospital, Huzhou 313099, Zhejiang Province, China
Wei Zhou: Department of Anesthesiology, Huzhou Central Hospital, Huzhou 313099, Zhejiang Province, China
Yinqiu Tan: Department of Neurosurgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430060, Hubei Province, China
Daofeng Tian: Department of Neurosurgery, Wuhan University, Renmin Hospital, Wuhan 430060, Hubei Province, China; Corresponding author.
Chunlong Zhong: Department of Neurosurgery, Shanghai East Hospital, School of Medicine, Tongji University, Shanghai 200120, China; Corresponding author.

Journal volume & issue: Vol. 8, no. 10
p. e11022

Abstract

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Gliomas typically have unfavorable prognosis, due to late detection and interventions. However, effective biomarkers for early glioma diagnosis based on 5-hydroxymethylcytosines (5 hm C) in circulating cell-free DNA (cfDNA) are not currently available. 5 hm C profiles in GSE132118 set were subjected for establishment of diagnostic model using the LASSO (least absolute shrinkage and selection operator) algorithm. The 5 hm C-based models demonstrated great potency in differentiating healthy subjects from gliomas, with area under the curves (AUCs) > 0.91 in the training and validation sets. Moreover, the indicator performed well in combination with clinicopathological characteristics to differentiate glioblastomas (GBMs) from lower grade glioma (LGGs). Enrichment analysis on 5 hm C profiles displayed great correlation with glioma pathophysiology. The 5 hm C-derived biomarker might act as an effective and non-invasive measure in glioma screening.

Published in Heliyon

ISSN: 2405-8440 (Online)
Publisher: Elsevier
Country of publisher: United Kingdom
LCC subjects: Science: Science (General); Social Sciences: Social sciences (General)
Website: https://www.cell.com/heliyon/home

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