Rapid generation of murine haploid-induced trophoblast stem cells via a Tet-on system

Abstract

Summary: Haploid trophoblast stem cells (TSCs) are advanced in studying placental development for their placental precursor and homozygous features. Here, we describe how to generate haploid-induced TSCs (haiTSCs) from haploid embryonic stem cells with a Tet-on system. Our haiTSCs can maintain haploidy long-term and can produce genome-wide mutants combined with transposons. It is promising in high-throughput genetic screening of trophoblast-specific modulators.For complete details on the use and execution of this protocol, please refer to Peng et al. (2019).

Keywords

• Cell Biology
• Developmental biology
• Genetics
• High Throughput Screening
• Stem Cells
• Cell Differentiation