Molecular characterization of Klebsiella pneumoniae associated with Thalassemia in Thi-Qar Governorate

Abstract

Klebsiella pneumoniae is a gram-negative, aerobic, non-motile bacilli and is a common cause of a wide range of infections in humans, Klebsiella spp. as a major causative bacteria in Thalassemia patient. The present study aimed to shed light on the supposed role of bacterial infection in Thalassemia patients during the period of from August 2022 to December 2023 in Genetic Blood Diseases/Thi-Qar Governorate. Forty blood sample were collected from all patients and subjected to Conventional Polymerase Chain Reaction assay by using the Universal gene. Nine PCR product were selected and subjected to partial DNA sequencing for the 16S rRNA gene to follow up their possible relationship between them and what recorded globally in Gene bank. The results revealed that 9/40(25%) isolates were K. pneumoniae. PCR product of 16SrRNA registered in Gene bank under the official accession numbers of OQ928953.1,OQ929666.1,OQ928950.1,OQ929250.1,OQ927231.1,OQ928942.1,OQ927249.1,OQ927229.1 and OQ927253.1). The phylogenetic tree that was constructed by MEGA-10 software showed that there were different molecular relationships among the local K. pneumonia isolates with analogous ones around the world.

Keywords

• Thalassemia, Gene sequencing, phylogenic tree