Influence of 6-BAP, period and subculture number in <em>Ruta graveolens in vitro</em> multiplication

Abstract

The rue (Ruta graveolens, L) it is a medicinal plant with grateful therapeutic properties, it has been found as scarse, into the twenty medicinal plants not abundant in Guantánamo province, Cuba. Before this situation the techniques of the tissue cultivation constitute a solution to maintain the environment biodiversity. For the establishment of a propagation methodology in vitro, it necessary the study of different factors that influence in the generation process development of the new plant; for the reason the present research was development in Centro de Desarrollo de la Montaña with the objective to study the concentration of 6-BAP, the period and the subcultivation number for the multiplication of Ruta graveolens in vitro through the direct organogenesis. Were used nodal segments with an axillary buds. The results showed that the multiplication of nodal segments of rue in MS cultivation supplemented with 25g.l-1 of sucrose, without 6-BAP, with four subcultivation every 21 days, it allowed a higher multiplication coefficient. Key words: in vitro multiplication, MS, organogenesis, rue